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KMID : 0895820090190040059
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Journal of Oriental Rehabilitation Medicine
2009 Volume.19 No. 4 p.59 ~ p.78
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Effects of Stem Bark Extracts of Cornus walteri Wanger on the Lipid Lowering, Anti-oxidative Activity and Concentration of Proinflammatory Cytokines in Rat Fed High Fat Diet
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Park Won-Hyung
Cha Yun-Yeop
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Abstract
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Objective: This study was designed to examine the effects of stem bark extracts of Cornus walteri Wanger on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines in hyperlipidemic rat.
Methods: Male rats weighing 195.21±5.85 g fed high fat diet for 8 weeks and 40 rats (above 400 g)were divided into 4 groups. Each groups were divided into a control group and 3 experimental groups. We fed a control group of rats a basal diet and administered normal saline (100 ㎎/㎏, 1 time/1day) for 4 weeks. And we fed each experimental group of rats Basal diet and administered an extract of Cornus walteri Wanger (100 ㎎/㎏, 200 ㎎/㎏, 300 ㎎/㎏, 1 time/1day)for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid of plasma and liver, concentration of proinflammatory cytokines, anti-oxidative activity and gene expression
Results: 1. Concentration of plasma free fatty acid, LDL-cholesterol showed a tendency to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma triglyceride, total cholesterol showed a significantly decrement in all Cornus walteri Wangerext. group than that of control group. HDL-cholesterol showed a significantly increment in the 300 ㎎/㎏ Cornus walteri Wanger ext. group. 2. Concentration of liver total cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of triglyceride liver showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group. 3. Concentration of plasma and liver TBARS showed a tendence to decrease in Cornus walteri Wanger ext. groups. The values of GSH-Px, SOD and CAT activity showed a significantly increment in the 300 ㎎/㎏ Cornus walteri Wanger ext. group than that of control group. 4. The values of plasma AST and ALT activity showed no significantly different in all treatment groups. 5. Concentration of plasma IL-1β, IL-6 and TNF-α showed a tendency to decrease in the Cornus walteri Wanger ext. groups. However the concentration of IL-10 in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly increment than that of control group. Concentration of liver IL-1β, TNF-α and IL-10 showed no significantly difference in all treatment groups. However concentration of IL-6 in the 200
and 300 ㎎/㎏ Cornus walteri Wanger ext. groups showed a significantly decrement than that of control group. 6. In the analysis of RT-PCR, gene expression of TNF-α, Apo-B, Apo-E and leptin in the Cornus walteri Wanger ext. groups showed a lower expression than that of control group. 7. The ratio of TNF-α, Apo-E and leptin expression per β-actin expression in the 200 and 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group. The ratio of Apo-B expressionper β-actin expression in the 300 ㎎/㎏ Cornus walteri Wanger ext. showed a significantly decrement than that of control group.
Conclusions: According to above results, in lowering lipid effect, antioxidative activity and antiinflammatory effect, the Cornus walteri Wanger ext. gives positive effect.
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KEYWORD
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CornuswalteriWanger, Cytokines, Loweringlipideffect, Antioxidativeactivity, Antiinflammatoryeffect
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